Phylogram showing the genetic relationships between the studied <i>Cryptococcus gattii</i> isolates.

<p>Dendrogram based on the maximum likelihood analysis of the concatenated seven ISHAM consensus MLST loci (8) using the program MEGA version 5.05 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105076#pone.0105076-Tamura1" target="_blank">[51]</a>. Bootstrap values higher than 50 are shown above the branches. Source, country, major molecular type, sequence type (ST), mating type and virulence of the strains in the <i>Galleria</i> model are indicated. Colour of the labels indicates clinical (black), veterinary (red) and environmental (green) strains; * mating type a, all others are mating type alpha; two letter country abbreviation (AR = Argentina, AU = Australia, BR = Brazil, CA = Canada, CO = Colombia, ES = Spain, IN = India, IT = Italy, NZ = New Zealand, PG = Papua New Guinea, TH = Thailand, US = USA, and ZA = South Africa); crosses represent more virulent (+++), similarly virulent (++), and less virulent strains (+), compared with the highly virulent VGIIa Vancouver Island outbreak strain CDCR265. Dash represents avirulent strains.