Effect of truncation on the homo-oligomerization capacity of Pmr.

<p>(A) Schematic representation of Pmr derivatives. Putative domain organization and a set of C-terminally or N-terminally truncated forms of Pmr, characterized in this study, are shown. Numbers indicate positions of amino acids. (B)–(G) Tricine-SDS-PAGE profiles of Pmr_nt₅₅ (B), Pmr_nt₆₁ (C), Pmr_nt₇₃ (D), Pmr (E), Pmr_ct₅₈ (F), and Pmr_ct₄₆ (G) after chemical cross-linking. The analyses were performed using DMS as a cross-linker. Numbers indicate incubation durations (min) with DMS (B–E) or concentrations (mg/ml) of DMS (F, G). “M” indicates the protein marker. The bands corresponding to oligomers, dimers, and monomers are indicated.