Design of experiments.

<p>Overview of experiments, where n denotes the number of experiments of a given type. In the “<i>E. coli</i> single cells” column, the box that straddles both the “Microfluidic” and the “Tube” fields corresponds to the method of carrying out a first round of amplification in a microfluidic chamber and then a second round of amplification in a test tube. This method will be denoted by “microfluidic+tube” in subsequent figure captions.