Mechanism of HNF4α-mediated improved hepatic differentiation.

<p><i>(</i><b><i>A</i></b><i>)</i> Detection of liver-enriched transcription factors in induced and HNF4α-transfected HuMSCs by RT-PCR. 0 d: HuMSCs before induction; DM 9 d and 21 d: Cells cultured in hepatic differentiation medium for nine days and 21 days, respectively; HNF4α 12 d and 21 d: Cells transfected with HNF4α nine days after induction (gene expression was determined three and 12 days later, respectively). <i>(</i><b><i>B</i></b><i>)</i> Wnt/β-catenin signaling-related genes were examined by real-time RT-PCR. All of the data are presented as the means ± SD (n = 3), and the fold induction for each gene by HNF4α overexpression was significant (P<0.05).