Antibody profiling after <i>B. pertussis</i> infection.

<p>Antibody responses were determined in mouse sera after intranasal infection by whole cell <i>B. pertussis</i> ELISA or MIA and expressed as OD450 or Fluorescent Intensity (F.I.) respectively. (A) IgM against OMV B1917 were absent. (B) Whole cell <i>B. pertussis</i> ELISA indicated IgG antibody formation 10–14 days p.i., which increased until day 28 days p.i. (C–G) Levels of total IgG and individual subtypes (IgG1, IgG2a, IgG2b and IgG3) against OMV B1917 indicated presence of multiple subclasses. (H) Anti-OMV IgA antibodies in serum were induced after 14–28 days p.i. (I) In lung lysates, anti-OMV IgA antibodies were detected 14 and 28 days p.i. I <i>p</i>-values were determined by one-way ANOVA with multiple comparison compared to naive mice (* = <i>p</i><0.05, ** = <i>p</i><0.01, *** = <i>p</i><0.001 and **** = <i>p</i><0.0001). (Mean of n = 3).