Impaired metabolic adaptation to fasting in SHIVA mouse.

<p>(A) Quantification of triglycerides in various lipoprotein fractions from fed or 16 hours fasted WT (filled bars) or SHIVA (clear bars) mice (n = 4 to 5 mice per condition). (B) Quantification of cholesterol in lipoprotein fractions (n = 5 to 8 mice per condition). (C) Quantification of triglycerides in liver from fed or 24/48 hours fasted mice (n = 4 mice per condition, representative experiment from 2 independent ones). (D) Glycemia, free fatty acids (FFA) and ketone bodies (beta hydroxyl butyric acid, bHBA) were measured after 14 h or 48 hours fasting. (E) Analysis of liver steatosis on oil red O stained OCT-frozen liver sections from carboxymethylcellulose (CMC) or fenofibrate (FIB)-treated-mice and (F) quantification using the ImageJ software by measuring the surface of the stained areas among 10 randomly-chosen fields per liver (3 mice per condition). Statistical analysis was performed using a two-way ANOVA test. (G) Pantetheinase activity (pAMC substrate) in serum or liver extracts from CMC (n = 3) versus FIB (n = 4) treated, WT or SHIVA mice.