Morpholino suppression of Pard3 causes variable cyclopia and loss of photoreceptor outer segments.

<p>(A–C) Transverse plastic sections of 4 dpf larvae heads from wild-type and <i>pard3</i> morphants show variable degrees of cyclopia but normal lamination. (D–F) Higher magnification of the boxed areas in A–C revealed the photoreceptor outer segments in wild-type retinas (Panel D, arrows), which were missing in <i>pard3</i> morphants. Morphants with fused eyes also exhibited patchy pigmentation in the RPE (Panel E, arrowheads). (G–H) Transmission electron microscopy of wild-type and <i>pard3</i> morphant retinas showed truncation of photoreceptor outer segments. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer. Scale bar = 50 µm (A–C); 10 µm (D–F); 1 µm (G–H).