Effects of fasting on expression and protein abundance of the <i>N</i>-reductive system in mice.

<p>Two groups of 12 C57BL/6W mice were fed with regular diet, one group were food deprived for 18 h (fasted) before sacrifice and liver collection, the second had full access to food and water (non-fasted). <b>A</b> Expressions of mARC1, mARC2, CYB5B, CYB5R and PCK1, determined with qRT-PCR, normalized on geometric mean of Ct values for Mcoln1 (Mucolipin-1) and Hmbs (hydroxymethylbilane synthase). Statistical significance was assessed by the U-test. <i>p</i>-values <0.05 were considered significant; (**) <i>p</i>-value <0.001; (***) <i>p</i>-value <0.0001. <b>B</b> Protein levels of mARC1, mARC2, CYB5B, CYB5R and PCK1 examined by Western Blot. Each sample consisted of equal protein amount of two individuals.