Assay development and validation of the HCI-based RVFV assay.

<p>(<b>A</b>) IFA of RVFV Gn expression (green) in RVFV-infected or mock-infected HeLa cells (cytoplasm is stained red). (<b>B</b>) Scatter plot distribution of percentage of cells expressing RVFV Gn acquired from mock-infected (green) or RVFV-infected (red) HeLa cells and the evaluation of degree of separation between the two controls (Z′-factor). Data are derived from triplicate experiments performed on three separate days. Horizontal line represents the average percent value of RVFV-infected cells.