Stability analysis of four new candidate genes.

<p><i>Panel A</i> - List of the four genes identified by microarray analysis. Primers were designed on the sequence corresponding to the accession number. The presence of pseudogenes and introns was determined by BLAST search on the rat genome (April 2014). <i>Panel B</i> - Fold change expression of the four candidate genes in injured nerves relative to control uninjured nerve, according to three published microarray data. The ratios were obtained by comparing the normalized fluorescence intensity at different time points after injury, with the fluorescence intensity of the control nerve. Average fold change (fc) relative to control uninjured nerve at different time points was analyzed: 1 (fc 1/0) and 5 days (fc 5/0) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105601#pone.0105601-Kim1" target="_blank">[14]</a>, 7 days (fc 7/0) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105601#pone.0105601-ArthurFarraj1" target="_blank">[15]</a>, 3 (fc 3/0), 7 (fc 7′/0) and 14 (fc 14/0) days <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105601#pone.0105601-Barrette1" target="_blank">[16]</a>. <i>Panel C</i> - Box-plot graph of cycle threshold values of the four HKG in 12 samples. Graphs are represented as medians (lines), 25th percentile to the 75th percentile (boxes) and ranges (whiskers) for all sample. <i>Panel D</i> - Ranking of the genes according to their expression stability (increasing from bottom to top) calculated on 12 samples. <i>ANKRD27</i> and <i>RICTOR</i> are the more stable genes according to NormFinder, <i>RICTOR</i> and <i>UBXN11</i> according to geNorm.