Identification of the most stable genes in the injured peripheral nerve.

<p><i>Panel A</i> - Box-plot graph of cycle threshold values of five HKG (2 commonly used and 3 new candidates) analyzed in 40 samples. Graphs are represented as medians (lines), 25th percentile to the 75th percentile (boxes) and ranges (whiskers) for all sample. <i>Panel B</i> - Ranking of the genes according to their expression stability (increasing from bottom to top) calculated on 40 samples. <i>ANKRD27</i> and <i>RICTOR</i> are the more stable genes according to both NormFinder and geNorm programs. <i>Panel C</i> - Average expression stability value (M) of genes remaining during stepwise exclusion of the least stable gene, according to geNorm: the lower the M, the more stable the gene. <i>Panel D</i> - Pairwise variation (V) analysis to determine the optimal number of control genes required for accurate normalization; according to geNorm <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105601#pone.0105601-Vandesompele1" target="_blank">[5]</a> a cutoff of 0.15 for V was used and two genes are sufficient.