Phylogenetic analysis of isolates of Plum bark necrosis stem pitting-associated virus (PBNSPaV) based on the nucleotide sequences of their partial HSP70h gene (590 nt) (A) and complete coat protein gene (B).

<p>The CP sequences of 38 isolates obtained in this study, the partial HSP70 sequences of 41 isolates obtained in this study, and 18 Chinese isolates previously reported were included in the analysis. The six isolates for which the complete genome sequence are available from GenBank are shown with “▴”. The three isolates for which the complete genomes were sequenced in the present study are indicated by “•”. Sequences previously reported by our group and the corresponding sequences available from GenBank are identified by their GenBank accession number following their isolate names. The divergent HSP70h variants within isolate Pch-GS-3 and the CP variants within isolate Pch-XN-3 are indicated by black arrows. The trees were constructed using the neighbor-joining method. The numbers at the nodes indicate bootstrap support (1,000 replicates). Values below 60% are not shown. The scale bar represents 0.01 substitutions per site.