PI3K/Akt/mTOR gene expression in developing Tg(fli1:EGFP) zebrafish.

<p>(A) RT-PCR and (B) qPCR examined the mRNA levels of zebrafish <i>pik3ca, pik3r1, mTOR and akt1</i> genes. Embryos were harvested at 6, 13, 18 or 24 hours post fertilization (hpf) and eyes at 48, 72 or 120 hours post fertilization (hpf). (A). Relative expression, normalised to <i>18S rRNA</i>, of <i>pik3ca, pik3r1, mTOR and akt1</i> mRNA levels in developing zebrafish embryos (black columns) and eyes (white columns) expressed relative to the 6 hpf stage. Results are expressed as mean ± S.D. (<i>n</i> = 3), *P<0.05, **P<0.01.