Cytotoxicity assay with 5-Fluorouracil.

<p>0.05×10⁶ SHSY5Y cells were seeded in the RTCA E plate and cultured in the presence or absence of 10 mM ManNProp or ManNPent followed by treatment with 5-FU. A. Cytotoxicity induced by 5-FU towards the engineered cells was measured. B. ManNProp and ManNPent treated cells displayed significant increase in cytotoxicity at 10-fold reduced 5-Fluorouracil concentration compared to untreated cells. ** P<0.0001 for ManNProp and ManNPent treatment until 25 µM 5-Flurouracil.