Down-regulation of c-Abl by siRNA counteracts MX induced apoptosis in HeLa and CaSki cells.

<p>(A) HeLa and CaSki Cells were transfected with non-targeting or c-Abl siRNA (75 nM). Western blot analysis was performed to examine the effect of siRNA. Level of c-Abl was quantitated and corrected for β-actin level. Values are proportioned to levels of control siRNA for each cell line. (B) HeLa, and (C) CasKi cells were transfected with control or c-Abl siRNA. After treansfection cells were treated with MX (0.6 µM), imatinib (5 µM) and their combination for 48 h. Relative amount of surviving cells was determined by WST-1 assay. Results are from two independent experiments in triplicates. Data are shown as mean ± SD. * p<0.05, ** p<0.01, *** p<0.001. NS, not significant.