Ezh2 SET domain depletion caused blockade of the cellular differentiation in embryonic liver.

<p>A: Western blot analysis for Albumin (hepatocyte marker) in the CD45⁻ TER119⁻ non-hematopoietic liver cells from control (Rosa26::CreER(T2)^−/−;Ezh2^F/F mouse) and Ezh2 KO (Ezh2 SET domain depleted mouse; Rosa26::CreER(T2)^+/−;Ezh2^F/F mouse) at ED 18.5 after 3 days TAM injection (TAM; ED 10.5–12.5). B: Expression levels of hepatocytes related genes in the CD45⁻ TER119⁻ non-hematopoietic liver cells of the control and Ezh2 KO at indicated points were measured by qRT-PCR. Data are mean ± SD (n = 3). <i>P</i> values (asterisks) are from the Mann–Whitney <i>U</i>-test. *<i>P</i>− TER119⁻ non-hematopoietic liver cells of the control and Ezh2 KO. D: Significant enrichment of metabolism related liver functional GO terms for decreased genes upon Ezh2KO. Corrected <i>P</i> values of GO terms are shown.