Effect of <i>ΔdsbA</i> and <i>ΔdsbB</i> mutations on the expression of CsgA and CsgD.

<p><b>A</b>) Coomassie blue stained SDS-PAGE gel revealing increased CsgA production in <i>ΔdsbA</i> and <i>ΔdsbB</i> mutants when grown on LA without salt at 28°C at 24 hours of incubation. <b>B</b>) Immunoblot for CsgD shows increased level of CsgD for <i>ΔdsbA</i> and <i>ΔdsbB</i> mutants from the same bacterial cultures. Numbers underneath the lanes in <b>A</b>) and <b>B</b>) specifies the relative band intensities. The <i>csgD-lacZ</i> promoter fusion activity at 18 hours post inoculation from salt-less Luria Agar cultures (<b>C</b>) or from salt-less Luria broth cultures (<b>D</b>) grown at 28°C. Error bars indicate SEM. *** = p≤0.001; ** = p≤0.01; ns = not significant compared to respective wild type.