Effect of OWB extract and CA on the JNK, MAPK, IkB-α and MyD88 expression.

<p>Expression of (A) JNK, (B) MAPK, (C) IkB-α and (D) MyD88 were determined by the Western blot, using GAPDH as the internal control. The LPS (1 µg/ml) induced peritoneal macrophage(s) were treated with OWB (100 µg/ml) or CA (10 µg/ml), and after 24 h the protein extract from whole cell were harvested in buffer, containing 20 mM Tris (pH 7±0.5), 50 mM NaCl, 5% NP-40 and 0.05% DOC. The soluble fraction was separated by centrifugation, subjected to SDS-PAGE and blotted to pre-equilibrated PVDF membrane. The membrane was then blocked in 5% NFDM in 1X TBST, rinsed and incubated with specific antibody at 4°C overnight. Immunoblotting was performed with peroxidase-labelled specific antibodies and visualized by ECL Western blot detection kit. The average expression of NF-kB and MAPK was significantly higher in the LPS-induced macrophage, as compared to the control and OWB or CA co-treated group (** <i>P</i>