Caveolin-1 knockdown recapitulate NOS3 dysfunction and leads to nitrate tolerance.

<p>(A) Representative measurement of basal NO and peroxynitrite level in WT and Cav-1 KO MECs. n = 6, ** <i>p<</i>0.01, *** <i>p</i><0.001 (B) Representative measurement of NO and peroxynitrite in WT and Cav-1 KO MEC stimulated with A23187 (10 µM) for 1 h. (C) Basal NO production in WT, Cav-1 knockout and Cav-1/NOS3 double knockout (DKO) MECs, n = 3 to 5, <i>* p</i><0.05, ** <i>p</i><0.01. (D) Basal Peroxynitrite production in WT, Cav-1 knockout and Cav-1/NOS3 double knockout MECs, n = 3 to 5, ** <i>p</i><0.01.