Expression of Caveolin-1 in Cav-1 knockout mouse endothelial cells eliminates peroxynitrite production.

<p>(A) NO and peroxynitrite measurement in Cav-1 -/- mouse endothelial cells transfected with either empty vector (EGFP) or wild type Cav-1-GFP construct. The production of NO and peroxynitrite was normalized by protein concentration. n = 6, ** <i>p</i><0.01. Cav-1 expression was analyzed and confirmed by western blot. (B) NO production in Cav-1 KO MECs treated with cell permeable caveolin-1 scafold domain (CSD, 25 µM) (C) Peroxynitrite production in Cav-1 KO MECs treated with cell permeable caveolin-1 scafold domain (CSD, 25 µM), n = 4, * <i>p</i><0.05, ** <i>p</i><0.01, results are representative of three independent experiments.