a<p>HSPB6 was diluted in buffer to a concentration of 1 mg/ml and the measurement was performed at 35°C.</p>b<p>0.25 mg/ml of insulin was incubated with 10 mM DTT at 37°C prior to measurement to assure full aggregation.</p>c<p>0.25 mg/ml insulin was incubated with sHSP in the indicated monomer molar ratio to yield the same concentrations as used for the chaperone assays. This mixture was incubated with 10 mM DTT at 37°C prior to measurement.</p><p>Size analysis by DLS of aggregated species.</p
<p><b>(A)</b> DTT-induced aggregation of 0.35 mg/ml insulin (Ins) in the absence and presence 0.35 m...
The kinetics of dithiothreitol (DTT)-induced aggregation of human recombinant insulin and the effect...
<p>(A) Size distribution profile of E7 dimer before addition EDTA. (B) Size distribution profile of ...
<p>Aggregation was monitored by following the absorbance at 340 nm for 90 min. The percentage protec...
(A) The tendencies of two insulin samples (0.5 mg/mL) were analyzed in the presence of DTT at 40°C. ...
The prevalence of diabetes is increasing every year making insulin formulations widely prescribed me...
<p>DLS measurements were repeated using 3 batch preparations with similar results.</p
<p>Aggregation of denatured protein substrates was followed by recording the optical density at 340 ...
<p>(A) Size-exclusion chromatography profiles, 100 µl of protein was loaded onto a Superdex 200 10/3...
Introduction: Insulin is a widely used antidiabetic drug, which regulates carbohydrate and fat metab...
abstract: Insulin is an essential peptide hormone that aides in the metabolism of glucose by allowin...
<p>(A) Size-exclusion chromatography profiles. 100 µl of protein was loaded onto a Superdex 200 10/3...
Size-exclusion chromatography (SEC) is the method of choice for the analysis of protein aggregates i...
Peak fronting occurs in size exclusion chromatography, SEC, resulting in 10% loss in insulin yield. ...
<p>DTT-induced aggregation of 0.35 mg/ml insulin at 25°C <b>(panel A)</b> and thermal aggregation of...
<p><b>(A)</b> DTT-induced aggregation of 0.35 mg/ml insulin (Ins) in the absence and presence 0.35 m...
The kinetics of dithiothreitol (DTT)-induced aggregation of human recombinant insulin and the effect...
<p>(A) Size distribution profile of E7 dimer before addition EDTA. (B) Size distribution profile of ...
<p>Aggregation was monitored by following the absorbance at 340 nm for 90 min. The percentage protec...
(A) The tendencies of two insulin samples (0.5 mg/mL) were analyzed in the presence of DTT at 40°C. ...
The prevalence of diabetes is increasing every year making insulin formulations widely prescribed me...
<p>DLS measurements were repeated using 3 batch preparations with similar results.</p
<p>Aggregation of denatured protein substrates was followed by recording the optical density at 340 ...
<p>(A) Size-exclusion chromatography profiles, 100 µl of protein was loaded onto a Superdex 200 10/3...
Introduction: Insulin is a widely used antidiabetic drug, which regulates carbohydrate and fat metab...
abstract: Insulin is an essential peptide hormone that aides in the metabolism of glucose by allowin...
<p>(A) Size-exclusion chromatography profiles. 100 µl of protein was loaded onto a Superdex 200 10/3...
Size-exclusion chromatography (SEC) is the method of choice for the analysis of protein aggregates i...
Peak fronting occurs in size exclusion chromatography, SEC, resulting in 10% loss in insulin yield. ...
<p>DTT-induced aggregation of 0.35 mg/ml insulin at 25°C <b>(panel A)</b> and thermal aggregation of...
<p><b>(A)</b> DTT-induced aggregation of 0.35 mg/ml insulin (Ins) in the absence and presence 0.35 m...
The kinetics of dithiothreitol (DTT)-induced aggregation of human recombinant insulin and the effect...
<p>(A) Size distribution profile of E7 dimer before addition EDTA. (B) Size distribution profile of ...