Recruitment of VEGFR1-positive cells from the BM, and hepatic I/R injury in BM chimeric mice.

<p>BM cells from GFP+WT mice (WT→WT) or GFP+VEGFR1 TK^-/- mice (VEGFR1 TK^-/- →WT) were injected into the tail vein of WT mice. (A,B) VEGFR1-positive cells (A) and double-positive cells (GFP- and VEGFR1-positive) (B) in the liver at 48 h post-reperfusion. (C) Expression of VEGFR1 and CD11b in the peripheral blood. Representative flow cytometry plots for GFP+WT BM (left panel) and GFP+VEGFR1 TK^-/- BM chimeric mice (right panel) are shown. (D) The percentage of BM-derived VEGFR1/CD11b-positive cells at 48 h at 48 h post-reperfusion.as assessed by FACS analysis. (E) Effect of transplanting BM cells from WT mice and VEGFR1 TK-/- mice on liver repair and hepatocyte proliferation at 48 h post-reperfusion. ALT levels, the area of hepatic necrosis, the hemorrhagic area, and the PCNA index were determined. Data are expressed as the mean ± SEM from six mice per group. *p<0.05 <i>vs</i>. GFP+WT BM chimeric mice.