Downregulation of HIF-1α reduces cellular invasion in uveal melanoma lines.

<p><b>A, B,</b> HIF-1α protein levels were determined by Western blot in OCM1 (A) and 92.1 cells (B) infected with HIF-1α or scramble shRNAs and exposed to normoxia or hypoxia for 24 hours. <b>C,</b> MTS assays were performed for 2, 4, 7 days in normoxia or hypoxia using OCM1 and 92.1 cells infected with HIF-1α or scramble shRNAs. P values were determined vs scramble control shRNA. <b>D, E,</b> Transwell invasion assay was carried out in OCM1 and 92.1 cells infected with HIF-1α or scramble shRNAs and exposed for 24 hours to normoxia or hypoxia (*p = 0.02; ***p = 0.0003).