ESI mass spectra to examine polymannuronic acid binding.

<p>ESI mass spectra acquired for aqueous ammonium acetate (50 mM) solutions of (A) <i>Pa</i>AlgX_27–474 (P) (4 µM) and 50 mM ManA₆); (B) <i>Pa</i>AlgX_27–474(P) (4 µM) and 50 mM ManA₁₂); (C) <i>Pa</i>AlgJ_79–379 (P) (10 µM) and 50 mM ManA₆); (D) <i>Pa</i>AlgJ_79–379 (P) (10 µM) and 50 mM ManA₁₂). A reference protein scFv (4 µM) was added into the solutions of AlgX, Lyz was added as P_ref to AlgJ solutions. Ion peaks corresponding to higher charge states of AlgJ are labelled as filled red circles. P_ref refers to the reference protein scFv.