The Swi5-Sfr1 activator complex blocks the Fbh1-mediated Rad51 dissociation from ssDNA.

<p>The ssDNA-bead assay was carried out as described in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004542#pgen-1004542-g001" target="_blank">Figure 1</a>. Intensity of Rad51 was quantified, and percentage reduction at each concentration of Swi5-Sfr1 was calculated as [(Rad51 amount on Beads in the presence of Fbh1)/(Rad51 amount on Beads in the absence of Fbh1)×100]. Upper panel shows the amount of Fbh1 in each lane. Each value and SE was obtained from three independent experiments.