Immunohistochemistry of PDSS2 in wild-type and silky-feather embryonic skins.

<p>Sections of wild-type (A–D) and silky-feather (E–H) skins at embryonic (E) days 9, 11, 14 and 17 are immunolabeled with the cPDSS2 antibody. PDSS2 staining is in green, and the nuclei are stained with DAPI (blue). (I) Schematic view of feather development and the sections corresponding to (A–H). The strong green signals that appear obviously unusual in the mesenchyme in (A and E) are suspected to originate from tissue damage caused by the experimental operation (according to bright field and not shown). Scale bar equals 100 µm.