FGK4.5 treatment enhances CD8⁺ T cell responses induced by VSV infection.

<p>C57BL/6 mice were i.v. infected with 2×10⁵ pfu VSV-ova and treated i.p. with 100 µg FGK4.5, an agonistic CD40 mAb, one day post infection. (A) Sixty days after primary infection the Ova/K^b-specific CD8⁺ memory T cell population in the blood was quantified by Ova/K^b-tetramer staining. (B) To assess whether activating CD40 would induce protective immunity, vaccinated mice were re-challenged with 5×10⁵ cfu LM-ova sixty day after VSV-ova vaccination. Three days later spleens and liver were assessed for bacterial burden. Statistical significance was determined using a one-way ANOVA with a Bonferroni’s post-test (**p<0.01). Data are representative of at least three independent experiments.