Expression of TSLPR and IL-7Rα in epidermal keratinocytes is controlled by ΔNp63.

<p>(<b>a</b>) Immunofluorescence labeling confocal microscopy to localize TSLPR and IL-7Rα in the area of epidermis where ΔNp63 was not detected. Bar = 20 µm. (<b>b</b>, <b>c</b>) Quantitative RT-PCR (<b>b</b>) and immunoblot analysis (<b>c</b>) against ΔNp63, TSLPR, IL-7Rα and TSLP in control siRNA (siControl) and p63-specific siRNA (sip63)-transfected HaCaT keratinocytes. β-actin was used as a loading control. The cells were harvested for assays at 72 hr after siRNA transfection. Student's <i>t</i> test. **<i>P</i><0.01 and N.S., not significant. (<b>d</b>) Immunofluorescence labeling fluorescence microscopy to localize TSLPR in siControl and sip63 HaCaT keratinocytes. The cells were fixed at 72 hr after siRNA introduction. DAPI, 4′,6-diamidino-2-phenylindole. Bar = 50 µm. Data are representative of at least three independent experiments.