Effect of sigmar-1 gene silencing on the DMT-modified cytokine profile of LPS or polyI:C-activated moDCs.

<p><b>A:</b> Validation of siRNA knockdown by Western blot. MoDCs were transfected with negative control siRNA (ctrl siRNA) or with gene-targeting siRNA (sigmar-1 siRNA), or left untreated (non-transfected, NT). <b>B–C:</b> Non-treated, 24 h ctrl siRNA-only, and 24 h targeting siRNA-only treated cells were used as negative controls (black bars). Red bars represent 24 h 500 ng/ml LPS-treated cells, while white bars show ctrl siRNA and 1 h DMT pre-treated cells activated with LPS for one day. Grey (NN-DMT) and checkered bars (5-MeO-DMT) demonstrate 1 h DMT pre-treated and then 24 h LPS activated sigmar-1 knockdown cells. <b>D–E:</b> MoDCs were treated as in Fig5B–C. Here, cell activation was performed with a 24 h 20 µg/ml polyI:C treatment. Blue bars represent polyI:C-only stimulation as positive control. Results are shown as Mean ± SEM of three independent donors. (*) represents <i>p</i> values <0.05. Differences are significant (<i>p</i><0.05) in all cases of specific activation (LPS or polyI:C) versus control cells (no treatment, ctrl siRNA, sigmar-1 siRNA).