Actin instability at the hyphal tip of a <i>Δ</i><i>Agrho5</i> mutant.

<p>DIC and fluorescence images of a hypha of a <i>ΔAgrho5</i> strain carrying an actin-binding domain fused to GFP. The growth of the strain was followed by time-lapse microscopy for 150 minutes. Images were taken every 5 minutes. For each time-point, pixel intensities were measured over the first 2 µm of the hypha. The difference between the pixel intensities of the upper half of pixels versus the lower half of the hypha is plotted for each time-point in the graph below the images. In addition, for each time-point, the corresponding hyphal tip is shown in heat map coloring, with cold colors representing low-pixel intensities and warm colors represent high-pixel intensities. The scale bar represents 10 µm.