Different treatments affect global levels of histone modification.

<p>(A) Western blot was performed using H3, H3ac, H3k9ac, H4K5ac and H3K4me2 antibodies in roots of maize seedlings exposed to different treatments. (B) Histogram showing the mean gray values of the western blot bands for histone modification. Error bars represent the standard error of the mean. The experiments were repeated three times using biologically independent samples. (C) Nuclei from seedlings roots under different treatments for 24h were immunostained (H3K9ac, H4K5ac and H3K4me2 panels) and counterstained with DAPI (DAPI panel). The ‘Merge’ panel shows a merged image of blue and green staining. More than 200 nuclei were analyzed for every antibody. Bar = 10 µm.