oxLDL characterization and citotoxicity assay.

<p><b>A)</b> oxLDL obtained from human plasma was oxidized with 7 uM of CuSO₄, monitored by spectrophotometry at λ 243 nm and electrophoresed in 1% agarose gels with sodium borate buffer. <b>B)</b> Cytotoxicity assay of prostate cancer cell models treated with 25, 50, and 100 µg/mL oxLDL during 12 hours. The data represent the means ± S.D. of three independent experiments performed in triplicate and statistically analyzed using one-way ANOVA and Dunnett’s post-test (* or # <i>p≤0.05</i>).