Seed biomass for WT and transgenic plants.

<p>(<b>A</b>) Phenotype of WT, <i>esk1i</i>-3, <i>esk1i</i>-<i>ICE1</i>-7, <i>esk2i</i>-<i>ICE1</i>-6, <i>CBF</i>-3, and <i>ICE1</i>-3 in normal watering environment. a, eight-week-old seedlings, b, six-week-old seedlings. (<b>B</b>) Average seed biomass. In the normal watering environment, SD (error bars) calculated from 30 plants of each phenotype and the results of three independent experiments, transgenic lines were as description in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106509#s4" target="_blank">methods and materials</a>. After drought treatment, SD (error bars) calculated from 10 plants of each phenotype and the results of three independent experiments, transgenic lines were as description in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106509#s4" target="_blank">methods and materials</a>. Asterisks indicate significant differences from the corresponding WT values determined by Student's <i>t</i>-tests (*0.01≤P≤0.05, **P≤0.01, ***P≤0.001). Experiments were repeated at least three times with similar results.