Flow cytometric analysis of CFTR expression in neutrophils and phagosomes.

<p><b>A</b>) CFTR staining in neutrophils. Purified peripheral-blood neutrophils were immunostained for intracellular CFTR using the monoclonal CFTR antibody (mAb #660). Representative dot plots show that the neutrophils from Myeloid-Cftr−/− mice express CFTR (93%), and the cells from the control Cftr^fl10 mice are 89% positive for CFTR. In contrast, the isotype control antibody shows little background staining. <b>B</b>) CFTR staining in phagosomes. Latex beads (3 µm) were phagocytosed by neutrophils from the control Cftr^fl10 mice or the Myeloid-Cftr−/− mice. Phagosomes released by cell homogenization were immunostained with the same anti-CFTR antibody. The phagosomal population was gated by flow cytometry. Only the phagosomes from the control Cftr^fl10 neutrophils show significant staining for CFTR.