Activation of RhoA restores the contractile capabilities of CD9 shRNA HAOSMC and partially restores actin arrangement.

<p><b>A</b> and <b>B,</b> GTP-bound (active) and total RhoA was quantitated using whole cell lysates of Ctr shRNA HAOSMC or CD9 shRNA HAOSMC treated with the RhoA activator CN03 (2.0 µg/ml) for two h (n = 3; NS = not significant; **<i>P</i><0.01). <b>C</b> and <b>D,</b> CD9 shRNA cells were treated with CN03 (2.0 µg/ml) for two h prior to releasing the sides of a collagen gel contraction assay. A representative image of a collagen gel contraction taken at 6 h and quantification of collagen gel contraction from 0-6h is shown (n = 3/time point). <b>E,</b> A representative immunofluorescent stained image of f-actin arrangement from three independently repeated experiments in untreated Ctr shRNA and CD9 shRNA treated with 2.0 µg/ml of CN03 for two h (scale bar, 50 µm). <b>F,</b> Actin filament length was quantified using ImageJ analysis software (n = 15 cells; *<i>P</i><0.05).