Generation and Characterization of a Transgenic Pig Carrying a DsRed-Monomer Reporter Gene - Figure 8

<p>(a) Cytometric analyses of CD4a, CD31, CD44, and CD90 conjugated with FITC on DsRed pig amniotic fluid progenitor/stem cells (pAFPCs). pAFPCs were negative for the surface antigens CD4a and CD31, and positive for CD44 and CD90. (b) Mesoderm trilineage differentiation potential of DsRed pAFPCs. DsRed pAFPCs exhibited spindle-shaped morphology (A1) and expressed red fluorescence under fluorescent microscopy (A2). Lipid droplets were observed in the DsRed pAFPCs adipogenic differentiation culture by using Oil-Red O staining (B1). Calcium accumulation was observed in the osteogenic differentiation culture by using Alizarin red staining (C1). Collagen was detected in the chondrogenic differentiation culture by using toluidine blue staining (D1). Under fluorescent microscopy, red fluorescence was still evident after osteogenetic (B2), adipogenetic (C2), and chondrogenetic (D2) differentiation.