<p>Kc cells were transfected with the indicated reporters with or without a plasmid expressing Arm* and assayed for luciferase expression. (A) Synthetic W-CRMs containing two HMG-Helper site pairs in all four orientations with 0, 3, 6, 9 or 12 bp spacing between the HMG and Helper site (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004591#pgen.1004591.s011" target="_blank">Table S1</a> for sequences of each synthetic W-CRM). In every construct, the two pairs were separated by 6 bp. Two constructs containing only HMG sites (HMG1 and HMG2) were included in the analysis and the reporter vector without an insert is referred to as empty vector (EV). The data shown represents the average of three independent expe...
<p>(A) Box and whiskers plots of the relative luciferase expression from pGL3 reporter constructs tr...
<p>(A) Schematic representation of pFLAG-Bach1 (WT) and pFLAG-Bach1 (AP4-7) used in these experiment...
BackgroundFluorescent reporters are useful for assaying gene expression in living cells and for iden...
<p>(A) Previously characterized W-CRMs <a href="http://www.plosgenetics.org/article/info:doi/10.1371...
<p>(A) Competition electromobility shift assay (EMSA) experiments performed with a recombinant TCF f...
<p>The different FBs were either co-cultured with themselves or with NCI-H1437 or Calu-1 tumor cells...
Common genome-editing strategies are either based on non-homologous end joining (NHEJ) or, in the pr...
<p>A. Composition of the KBS reporter constructs. The 4×KBS reporter contains four consensus Kaiso b...
Common genome-editing strategies are either based on non-homologous end joining (NHEJ) or, in the pr...
(A) Scheme of the luciferase reporter construct, derived from the natural HSPA1A promoter, with Nano...
<p>(A) GFP reporter construct for assessment in different cell lines. The reporter plasmid is the sa...
<p><b>A.</b> Plasmid constructs for luciferase reporter assays. The empty modified pcDNA3.1 was used...
Background: Fluorescent reporters are useful for assaying gene expression in living cells and for id...
<p>(A) Schematic of CMV promoter-driven luciferase reporter genes containing pentamers that encode e...
<p>A. The composition of the full-length <i>MMP-7</i> reporter (HMAT-2.3). The HMAT-2.3 reporter con...
<p>(A) Box and whiskers plots of the relative luciferase expression from pGL3 reporter constructs tr...
<p>(A) Schematic representation of pFLAG-Bach1 (WT) and pFLAG-Bach1 (AP4-7) used in these experiment...
BackgroundFluorescent reporters are useful for assaying gene expression in living cells and for iden...
<p>(A) Previously characterized W-CRMs <a href="http://www.plosgenetics.org/article/info:doi/10.1371...
<p>(A) Competition electromobility shift assay (EMSA) experiments performed with a recombinant TCF f...
<p>The different FBs were either co-cultured with themselves or with NCI-H1437 or Calu-1 tumor cells...
Common genome-editing strategies are either based on non-homologous end joining (NHEJ) or, in the pr...
<p>A. Composition of the KBS reporter constructs. The 4×KBS reporter contains four consensus Kaiso b...
Common genome-editing strategies are either based on non-homologous end joining (NHEJ) or, in the pr...
(A) Scheme of the luciferase reporter construct, derived from the natural HSPA1A promoter, with Nano...
<p>(A) GFP reporter construct for assessment in different cell lines. The reporter plasmid is the sa...
<p><b>A.</b> Plasmid constructs for luciferase reporter assays. The empty modified pcDNA3.1 was used...
Background: Fluorescent reporters are useful for assaying gene expression in living cells and for id...
<p>(A) Schematic of CMV promoter-driven luciferase reporter genes containing pentamers that encode e...
<p>A. The composition of the full-length <i>MMP-7</i> reporter (HMAT-2.3). The HMAT-2.3 reporter con...
<p>(A) Box and whiskers plots of the relative luciferase expression from pGL3 reporter constructs tr...
<p>(A) Schematic representation of pFLAG-Bach1 (WT) and pFLAG-Bach1 (AP4-7) used in these experiment...
BackgroundFluorescent reporters are useful for assaying gene expression in living cells and for iden...