Bioinformatics analyses of siRNA screen hits.

<p>(A) Venn diagram of siRNA hits identified in this work. We identified 41 host proteins in HIV screen and 52 host proteins in M-PMV screen. There were 24 host proteins that were common for both HIV-1 and M-PMV. (B) Protein interaction network of HIV1 screen hits (Stronger associations are represented by thicker lines). A map of the interactions of our HIV screen hits was built by using STRING 9.05 with a required medium confidence score (0.400). Analysis of the network revealed clusters of interrelated factors involved in three distinct pathways (highlighted in grey circle): cellular actin cytoskeletal regulation pathways, clathrin-mediated trafficking factors and regulators of phosphoinositide metabolism. (C) Protein interaction network of the common screen hits for both HIV-1 and M-PMV (Stronger associations are represented by thicker lines). The interactions map of 24 host proteins that were common for both HIV-1 and M-PMV was built by using STRING 9.05 with a required medium confidence score (0.400). Factors involved in clathrin-mediated trafficking, regulation of the actin cytoskeleton, and phosphoinositide metabolism (highlighted in grey circle) were identified to affect both HIV-1 and M-PMV assembly or release.