Vascular leakage corresponds to disturbances in the outer plexiform and outer nuclear layers of the retina.

<p>Images showing the representative time course of lesion development in a NRV2 mouse. (<i>A</i>–<i>C</i>) Fundus image taken at p17, showing areas that have slight depigmentation, at the 1 o’clock position of the image (<i>A</i>, left panel). In the same area, vascular leakage can be seen by fluorescein angiography (<i>B</i>, middle panel). Examination by SD-OCT at the region of vascular leakage (red bar in <i>B</i>) reveals a disturbance in the ONL and OPL of the retina (<i>C</i>, right panel). Arrowheads point to the retinal disturbance. (<i>D</i>–<i>F</i>) Fundus image taken at p21, of the same mouse, shows increased depigmentation at the 1 o’clock position of the image (<i>D</i>, left panel). In the same area vascular leakage can be seen by fluorescein angiography (<i>E</i>, middle panel). Examination by SD-OCT at the region of vascular leakage (red bar in <i>E</i>) reveals a disturbance in the ONL and OPL of the retina (<i>F</i>, right panel). Arrowheads point to the retinal disturbance. (<i>G</i>–<i>I</i>) Fundus image of the same mouse taken at p25, the height of leakage in NRV2 mice, shows depigmentation, at the 1 o’clock position of the image (<i>G</i>, left panel). In the same area, vascular leakage is pronounced compared to the earlier time points as seen by fluorescein angiography (<i>H</i>, middle panel). Examination by SD-OCT at the region of vascular leakage (red bar in <i>H</i>) reveals a disturbance in the ONL and OPL of the retina (<i>I</i>, right panel). Arrowheads point to the retinal disturbance. p = postnatal day, GC: ganglion cells; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; IS/OS: photoreceptor inner segment/outer segments. n = 10, Representative images are shown. Scale bars: 50 µm.