Mutations induced by Cas9/gRNAs in goat fibroblasts.

Wei Ni (286780)
Jun Qiao (328589)
Shengwei Hu (392403)
Xinxia Zhao (625584)
Misha Regouski (625585)
Min Yang (146229)
Irina A. Polejaeva (504731)
Chuangfu Chen (328590)

Publication date

September 2014

DOI

10.1371/journal.pone.0106718.g001

Abstract

<p>(A) Design and activity of Cas9/gRNAs in goat fibroblasts. The restriction sites in the target regions are bold. The PAM sequence is underlined. (B) Cas9/gRNA-induced mutations in MSTN, PrP, BLG and NUP genes. The sizes of the deletions (−) and insertions (+) are shown to the right of each allele. Insertions are lower case.</p