Virus control and type I IFN responses of myeloid cells derived from TLR3^−/− and TLR4^−/− mice to JEV infection.

<p>Primary bone marrow-derived DCs (BMDC) and macrophages (BMDM) recovered from TLR3^−/− and TLR4^−/− mice were infected with JEV at a MOI of 1.0 for viral replication and 10 for cytokine expression. <b>A</b>. JEV replication in BMDC and BMDM. Viral RNA replication was expressed by viral RNA copy number per microgram of total RNA. <b>B</b> and <b>C</b>. The expression of pro-inflammatory cytokines (IL-6 and TNF-α) in infected BMDC and BMDM. <b>D</b> and <b>E</b>. The expression of type I IFNs (IFN-α and β) in infected BMDC and BMDM. <b>F</b>. The secretion of IFN-β protein by infected BMDC and BMDM. The mRNA levels of the indicated cytokines were determined by real-time qRT-PCR and the cytokine levels in culture media were determined by ELISA. Data represent the average ± SD derived from BMDC and BMDM evaluated in quadruplicate. *, <i>p</i><0.05; **, <i>p</i><0.01; ***, <i>p</i><0.001 compared with the levels of the wild-type control.