MB231_CFP-YFP cells retain sensitivity to TRAIL and anti-DR5 antibody.

<p>(A) Parental MB231 (filled black) and MB231_CFP-YFP (open clear) cells were treated with TRAIL (0–100 ng/ml) for 2.5 h or anti-DR5 antibody (0–200 µg/ml) for 20 h, at the indicated concentrations. Cell viability was determined by MTT assay. (B) Cells were treated as in A and analyzed for apoptosis by flow cytometry after staining with Annexin-V-APC and propidium iodide (PI). p-values were determined using a Student’s t-test. EC₅₀ values were determined using nonlinear curve fitting as described in the Materials and Methods section. For each EC₅₀ value, 95% confidence intervals reflecting the statistical accuracy are reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107010#pone-0107010-t001" target="_blank">Table 1</a>.