Anti-CD25 mAb administration reduced CD4⁺ T cells in mouse liver, not in blood or spleen.

<p>After one hour reperfusion, fleshly obtained hepatic nonparenchymal cells were isolated from PC61 or control IgG administrated C57Bl/6 mice. (A) Representative flow cytometry results from anti-CD3 and anti-CD4 stained hepatic nonparenchymal cells. (B) Total cells were gated on the 7-AAD⁻CD45⁺ before the FACS analysis, CD4⁺ (CD3⁺/CD4⁺), CD8⁺ (CD3⁺/CD8⁺) T cells, NK (CD3^–/NK1.1⁺), NK T(CD3⁺/NK1.1⁺) cells, B cells (B220⁺/CD19⁺), neutrophils (GR-1⁺/CD11b⁺), Kupffer cells (F4/80^low/CD11b⁺), or dendritic cells (F4/80^high/CD11b⁺) were compared in each group. Data are mean values±SE; n = 5 per group. (***p<0.001).