<p>(A) Effect of pH on β-glucosidase activity. The enzyme assay was performed at 80°C for 10 min. (B) pH stability. The enzyme was pre-incubated without substrate at 37°C for 60 min, and then subjected to residual activity assay under standard conditions (pH 5.0, 80°C, 10 min). (C) Effect of temperature on β-glucosidase activity determined at pH 5.0 for 10 min. (D) Thermostability. The residual enzyme activities were measured under standard conditions after pre-incubation of the enzyme without substrate in McIlvaine buffer (pH 5.0) for various periods. Each value in the panel represents the means ± S.D. (<i>n</i> = 3).</p
<p>(a) Effects of temperature, from 20°C to 45°C, on enzyme activity in the presence of Mg<sup>2+</s...
<p><b>A</b> and <b>B:</b> The recombinant xylanase incubated in various buffers (pH 3–12) and temper...
<p><b>A</b>. The optimal pH. Enzyme activity was determined with 100 mM glyphosate at 25 °C and with...
<p>a. The effect of pH on lipase activity. The activity assay was performed at 37°C in buffers of pH...
242-247This study presents isolation and characterization of β-glucosidase (BG) from a bacterial iso...
<p>(A) Effect of pH on <i>β</i>-1,4-glucanase activity. (B) Effect of pH on the stability of <i>β</i...
<p>(<b>A)</b> Effect of pH on enzyme activity assayed at 60°C. (<b>B)</b> pH stability. The enzyme a...
<p>The optimal temperature was determined by measuring the enzyme activity at selected temperatures ...
<p>(a) Effect of pH on enzymatic activity, measured in 50 mM glycine-HCl buffer (pH 2.5–3.5, closed ...
<p>The residual activity was measured after 4(B) and <i>o</i>NPG was used as substrate for the enzym...
<p><b>A</b> Effect of pH on β-glucanase activity of BGL7AM and BGL7A at 60°C. <b>B</b> pH stability ...
<p>The maximum value obtained was set to 100%. (A) Optimal pH of <i>L. migratoria</i> GSTs assayed u...
<p>(A) The effects of temperature. (B) The effects of buffer and pH value. All the reaction mix (100...
A GH3 β-glucosidase (BGL) from Penicillum brasilianum was purified to homogeneity after cultivation ...
<p>The assay was performed in the presence of 10 mM NaTC. While the WT enzyme has a pH optimum of 5....
<p>(a) Effects of temperature, from 20°C to 45°C, on enzyme activity in the presence of Mg<sup>2+</s...
<p><b>A</b> and <b>B:</b> The recombinant xylanase incubated in various buffers (pH 3–12) and temper...
<p><b>A</b>. The optimal pH. Enzyme activity was determined with 100 mM glyphosate at 25 °C and with...
<p>a. The effect of pH on lipase activity. The activity assay was performed at 37°C in buffers of pH...
242-247This study presents isolation and characterization of β-glucosidase (BG) from a bacterial iso...
<p>(A) Effect of pH on <i>β</i>-1,4-glucanase activity. (B) Effect of pH on the stability of <i>β</i...
<p>(<b>A)</b> Effect of pH on enzyme activity assayed at 60°C. (<b>B)</b> pH stability. The enzyme a...
<p>The optimal temperature was determined by measuring the enzyme activity at selected temperatures ...
<p>(a) Effect of pH on enzymatic activity, measured in 50 mM glycine-HCl buffer (pH 2.5–3.5, closed ...
<p>The residual activity was measured after 4(B) and <i>o</i>NPG was used as substrate for the enzym...
<p><b>A</b> Effect of pH on β-glucanase activity of BGL7AM and BGL7A at 60°C. <b>B</b> pH stability ...
<p>The maximum value obtained was set to 100%. (A) Optimal pH of <i>L. migratoria</i> GSTs assayed u...
<p>(A) The effects of temperature. (B) The effects of buffer and pH value. All the reaction mix (100...
A GH3 β-glucosidase (BGL) from Penicillum brasilianum was purified to homogeneity after cultivation ...
<p>The assay was performed in the presence of 10 mM NaTC. While the WT enzyme has a pH optimum of 5....
<p>(a) Effects of temperature, from 20°C to 45°C, on enzyme activity in the presence of Mg<sup>2+</s...
<p><b>A</b> and <b>B:</b> The recombinant xylanase incubated in various buffers (pH 3–12) and temper...
<p><b>A</b>. The optimal pH. Enzyme activity was determined with 100 mM glyphosate at 25 °C and with...