Connexin32 expression and junction formation augments the growth inhibitory effect of 1-25D.

<p>LNCaP-P, LNCAP-N and LNCaP-32 cells were seeded at clonal density (2×10³) and treated with 1-25D (1 nM) after 24 h. Cells were grown for 21 days with a medium change every 4 days when they formed colonies. Colonies were fixed and stained with crystal violet. <b>A</b>. Representative dishes showing colonies. <b>B</b>. Morphology of the individual colonies at higher magnification. Note the robust decrease in colony size in 1-25D-treated dishes.