Effect of 1,25D and androgen on the junctional transfer of fluorescent tracers in LNCaP-32 cells.

<p>LNCaP-32 cells, seeded in 6 cm dishes in replicate, were grown to 65–70% confluence. Junctional transfer was measured after microinjecting fluorescent tracers (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0106437#s2" target="_blank">Materials and Methods</a>).</p>a<p>The number of fluorescent cell neighbors (Mean ± SE) 1 min (Lucifer Yellow), 3 min (Alexa-488) and 15 min (Alexa-594) after microinjection into test cell. The total number of injection trials is shown in parentheses.</p>b<p>Cells were treated for 48 h with 1-25D, DHT (10 nM) and MB (2.5 nM).</p>c<p>P≤0.0001 for normal serum versus stripped and treated cells. A two tailed Student's <i>t</i> test was used to calculate P value assuming unequal variance.</p><p>Effect of 1,25D and androgen on the junctional transfer of fluorescent tracers in LNCaP-32 cells.