Effect of 1-25D and MB on the formation of gap junctions in the presence and the absence of Casodex.

<p>LNCaP-32 cells, seeded on glass cover slips, were grown to 70% confluence. Cells were then grown for additional 24 h in normal medium (NS), androgen-depleted medium alone (ST), normal serum supplemented with Casodex (CDX; NS+CDX), androgen-depleted medium supplemented with MB (ST+MB), MB and Casodex (ST+MB+CDX), 1-25D (ST+1-25D), 1-25D and Casodex (ST+1-25D+CDX) and in normal serum with 1-25D (NS+1-25D). Gap junction formation was assessed immunocytochemically. Note that GJs (green) are not degraded in cells treated with 1-25D both in the presence and absence of Casodex whereas they are degraded in normal serum and androgen-depleted but MB supplemented medium containing Casodex. E-cad is shown in red and the nuclei (blue) are stained with DAPI. Scale Bar = 20 µM.