Dissection of G6P-utilizing pathways by gene functional analysis.

<p>(<b>A</b>) Radial growth on complete media (CM) was not impaired in mutant strains carrying gene deletions in <i>FBP1</i>, <i>PGI1</i> or <i>TKL1</i>. Guy11 is the wild type isolate used in this study. (<b>B</b>) Sporulation was impaired in strains lacking functional <i>PGI1</i> and <i>TKL1</i> genes, but not <i>FBP1</i>, during growth on CM. Results are the mean of three or more independent measurements. Error bars denote SD. Bars with the same letters are not significantly different (<i>Student's t-test</i> p≤0.05). (<b>C</b>) Spores of Guy11, Δ<i>pgi1</i>, Δ<i>fbp1</i> and Δ<i>tkl1</i> were applied to artificial hydrophobic surfaces. At 24 hr post inoculation (hpi), the rate of appressorial formation by either gene deletion strain was not significantly different to Guy11. Error bars are SD. Bars with the same letter are not significantly different (<i>Student's t-test</i> p≤0.05). (<b>D</b>) Loss of <i>PGI1</i>, <i>FBP1</i> or <i>TKL1</i> did not impair the formation of melanized appressoria on artificial hydrophobic surfaces by 24 hpi. Scale bar is 10 µm.