<p>Shown are the average EC<sub>50</sub> values, SD and fold-change in EC<sub>50</sub> of PLY-mediated hemolysis of sheep RBCs after serial dilution into PBS containing 10% sera from the indicated mouse strains. The fold change = EC<sub>50</sub> in the presence of serum/EC<sub>50</sub> in PBS. Results from 3 independent experiments are shown. No PLY antibodies were detected in these sera (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004353#ppat.1004353.s003" target="_blank">Table S1</a>). Note: Non-Swiss albino serum for <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004353#ppat-1004353-t001" target="_blank">Table 1</a> was from a different pooled lot as that shown here.</p>...
<p>(A) Human erythrocytes were exposed to varying concentrations of purified recombinant VLY for 30 ...
<p>Hemolysis was analyzed with 1% sheep red blood cells suspension in PBS. Bovine serum albumin (BSA...
<p>A. Hemolysis on 5% sheep blood agar plates. (1) SH1000+pCU1, (2) SH1000:<i>msrA</i>+pCU1, (3) SH1...
<p>Shown are the average EC<sub>50</sub> values, standard deviation (SD) and fold-change in EC<sub>5...
<p>PLY requires the free 3β-hydroxyl of cholesterol to bind therefore treatment with CHOD will aboli...
<p>Hemolytic activity of Ply was measured in the presence of 2% RBCs. The results are shown as relat...
<p>Serum stimulated oligomer formation was determined by FRET between donor (D) and acceptor (A) lab...
<p>Effects of <i>potD</i> deletion on hemolytic activity were assessed via hemolysis assay, in which...
<p><b>A</b>: Inhibition of erythrocyte lysis in highly diluted (1.5%) serum with increasing concentr...
<p><b>A and B:</b> Sheep red blood cells were infected with <i>S</i>. Typhimurium SL1344 and derived...
<p>Left columns: Shown are the average EC<sub>50</sub> values, SD and fold-change in EC<sub>50</sub>...
<p>Balb/c mice were infected via i.v. route with 10<sup>6</sup> CFU of WT, WT:Ply-High or WT:Ply-Low...
The kinetics of aIternative-pathway mediated haemolysis were studied in sera from a number of verteb...
In this work the same hemolytic strain of B. coli (1192) was used that is described in the immediate...
<p>Different concentrations of rBiomphalysin, prepared by serial dilution of the Biomphalysin RTS 50...
<p>(A) Human erythrocytes were exposed to varying concentrations of purified recombinant VLY for 30 ...
<p>Hemolysis was analyzed with 1% sheep red blood cells suspension in PBS. Bovine serum albumin (BSA...
<p>A. Hemolysis on 5% sheep blood agar plates. (1) SH1000+pCU1, (2) SH1000:<i>msrA</i>+pCU1, (3) SH1...
<p>Shown are the average EC<sub>50</sub> values, standard deviation (SD) and fold-change in EC<sub>5...
<p>PLY requires the free 3β-hydroxyl of cholesterol to bind therefore treatment with CHOD will aboli...
<p>Hemolytic activity of Ply was measured in the presence of 2% RBCs. The results are shown as relat...
<p>Serum stimulated oligomer formation was determined by FRET between donor (D) and acceptor (A) lab...
<p>Effects of <i>potD</i> deletion on hemolytic activity were assessed via hemolysis assay, in which...
<p><b>A</b>: Inhibition of erythrocyte lysis in highly diluted (1.5%) serum with increasing concentr...
<p><b>A and B:</b> Sheep red blood cells were infected with <i>S</i>. Typhimurium SL1344 and derived...
<p>Left columns: Shown are the average EC<sub>50</sub> values, SD and fold-change in EC<sub>50</sub>...
<p>Balb/c mice were infected via i.v. route with 10<sup>6</sup> CFU of WT, WT:Ply-High or WT:Ply-Low...
The kinetics of aIternative-pathway mediated haemolysis were studied in sera from a number of verteb...
In this work the same hemolytic strain of B. coli (1192) was used that is described in the immediate...
<p>Different concentrations of rBiomphalysin, prepared by serial dilution of the Biomphalysin RTS 50...
<p>(A) Human erythrocytes were exposed to varying concentrations of purified recombinant VLY for 30 ...
<p>Hemolysis was analyzed with 1% sheep red blood cells suspension in PBS. Bovine serum albumin (BSA...
<p>A. Hemolysis on 5% sheep blood agar plates. (1) SH1000+pCU1, (2) SH1000:<i>msrA</i>+pCU1, (3) SH1...