The MMSET-induced epigenetic switch depends on multiple domains.

<p>(A) Diagram of the wild-type (amino acids 1–1365) and deletion MMSET constructs used for repletion experiments. Star represents location of the Y1118A mutation. –PHD construct encompasses amino acids 1–1209 and –PWWP2 construct is missing amino acids 877–995. (B) Representative immunoblot of nuclear extracts from repleted TKO cells was probed with the indicated antibodies. At least two independent infections were performed for each construct. (C) Representative qRT-PCR for <i>JAM2</i> in repleted TKO cells. Measurements were performed from multiple infections in at least duplicate and the graph represents average expression +/− standard deviation. (D) Methylcellulose colony formation assay using repleted TKO cells. Experiment was performed in triplicate and at least six different fields were counted. Graph represents average colony count +/− standard deviation. (E) ChIP assay on the <i>JAM2</i> promoter using repleted TKO cell. Two independent biological replicates are shown.